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Tuesday, June 23, 2015
5:45 pm - 8:45 pm | Dinner Workshop

RAPID SAMPLE PREPARATION FOR PATHOGEN DETECTION

This workshop will discuss sample preparation technologies for detection, identification and analysis of biomedical, biological and chemical agents, biothreats in point-of-care, laboratory and field settings. It will review the novel and rapid technologies for sample preparation, application of analytical strategies and automation in biodetection.

Topic to be covered:

  • Why efficient sample prep is critical in biodefense applications
  • Strategies for concentrating sample and faster pathogen recovery.
  • Development of qualification methods and protocols
  • Advances in sample prep- bridging the gap between advances in biodetection technologies and sample prep technologies
  • Review of high throughput sample prep technologies for clinical labs and R&D
  • Advances and automation in sample prep for point of care and point of need applications
  • Case studies on rapid sampling and sample preparation deployed for recreational water monitoring, biotech drug manufacturing, egg production, built environments, and wide area surveillance

Instructors:

Dave Alburty, CEO, InnovaPrep LLC
Anubhav Tripathi, Ph.D., Professor of Engineering, Professor of Molecular Pharmacology, Physiology and Biotechnology, Director of Biomedical Engineering, Brown University

Speaker Bios:

Dave Alburty 

Dave Alburty, CEO, InnovaPrep LLC 

Mr. Alburty has over twenty years experience in the Aerosol Research Industry. Beginning at Midwest Research Institute (MRI) in Kansas City his work as a staff research scientist centered on aerosol studies in environmental and biodefense applications. After serving fifteen years at MRI, Dave founded an aerosol science and engineering R&D test and evaluation/product development lab in Drexel Missouri. AlburtyLab offers third party validation to developers of many large-scale Homeland Security and Department of Defense projects as well as various commercial products.

Through his work in the biodefense industry, Dave recognized a missing technology between bio-collection and rapid biodetection/identification. That link is the advancement of sample preparation and biological concentration technologies. Through internal research and development projects at AlburtyLab, The InnovaPrep System was developed to fulfill that need. InnovaPrep LLC was subsequently launched in June 2009 as a manufacturer and integrator of biological sampling and concentration systems and solutions.

 

Anubhav Tripathi 

Anubhav Tripathi, Ph.D., Professor of Engineering, Professor of Molecular Pharmacology, Physiology and Biotechnology, Director of Biomedical Engineering, Brown University 

Dr. Tripathi is Professor of Engineering and Medical school at Brown University. Before joining Brown, he spent three years in Caliper LifeSciences (Now Perkin Elmer) where he developed microfluidics chips for protein and DNA sizing. Perkin Elmer now sells over million chips a year. At Brown, At Brown, his research program develops new pathogen diagnostic platforms by integrating biological and engineering principles. His group also performs transport modeling in biological systems. Dr. Tripathi also serves as a co-director for the Center of Biomedical Engineering, which is responsible for both undergraduate and graduate degrees in Biomedical Engineering. Dr. Tripathi received his PhD in Chemical Engineering from City University of New York. Thereafter he spent two years at Massachusetts Institute of Technology (MIT) as a postdoctoral associate.

 

Presentation Abstracts:


Anubhav Tripathi, Ph.D., Professor of Engineering, Professor of Molecular Pharmacology, Physiology and Biotechnology, Director of Biomedical Engineering, Brown University 

Fast and reliable diagnosis is an invaluble tool in bio-detection. In order to quickly and sensitively assess infection progression, monitor response to treatment and determine patient prognosis, patient samples (e.g, blood, urine, and saliva) are collected and analyzed for various biomarkers. Conventional sample processing entails many manual, time-consuming steps. Consequently, specimens must be processed by skilled technicians before detection of antigens or nucleic acids that are often present at dilute concentrations. Recently several automated technologies have been developed that can potentially offer many advantages over traditional bench-top extraction methods. The smaller length scales and more refined transport mechanisms that characterize these devices enable faster and more efficient biomarker enrichment and extraction. Additionally, their architectures can be designed to perform multiple tests or experimental steps on one integrated, automated platform. This workshop explores the strategies for concentrating sample and faster infection recovery from bench top to automated high throughput devices. Additionally, development of qualification methods and protocols covering a broad spectrum of sample preparation techniques and designs for various types of sample and species of analyte will be presented.

Dave Alburty, CEO, InnovaPrep LLC 

Rapid sampling and sample preparation deployed for recreational water monitoring, biotech drug manufacturing, egg production, built environments, and wide area surveillance will briefly be summarized and the similarities and differences will be discussed. Implications for human and animal health, as well as economic factors related to product quality control and will be included. This presentation will conclude with the significance of better sampling and sample prep for actionable decision-making in the cases presented.

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